Indirectly Excited Fluorescence Spectra

Simulation of x-ray absorption near-edge spectra and x-ray fluorescence spectra of optically excited molecules.

The x-ray absorption near-edge spectra (XANES) and fluorescence spectra of molecules in the ground state and optically excited states are computed using time-dependent density functional theory and time-dependent Hartree-Fock theory. The calculated XANES spectra of optically excited methanol, benzonitrile, hydrogen sulphide, and titanium tetrachloride and the fluorescence spectra of optically e...

Thermochromic Shifts of Absorption and Fluorescence Spectra and Excited State Dipole Moment of PRODAN*

PRODAN (Fig. 1) belongs to the category of solvent sensitive probes due to the fact that the aromatic naphthalene ring is attached to two groups: an electron donor and an electron acceptor which contribute to a distinct increase in the electric dipole moment A/u = /u,e-iug (/ie and is the dipole moment in the excited and ground state, respectively) as a result of excitation to the lowest excite...

On the Estimation of Excited-State Dipole Moments from Solvatochromic Shifts of Absorption and Fluorescence Spectra

The effect of the solvent polarity parameters f (e, n) and j (e, n) appearing in solvatochromic theories, and the effect of the molecular shape of the Onsager cavity (sphere, ellipsoid of revolution) on the determined electric dipole moments me in the singlet excited state are studied. It is found that the shape of the solute does not exhibit a significant effect on the determined values of me,...

Laser Excited Fluorescence of Dityrosine

Two-photon excited hemoglobin fluorescence

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. Thi...